PerfeCTa MultiPlex qPCR ToughMix
- 1-tube SuperMix minimizes pipetting steps, simplifying reaction assembly and improving accuracy
- 5x concentrated reagent provides more flexibility with dilute DNA samples sensitivity
- ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
- Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
- Supports efficient vortex mixing with proprietary anti-foaming additives
PerfeCTa Multiplex qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
PerfeCTa Multiplex qPCR ToughMix
PerfeCTa Multiplex qPCR ToughMix ROX
PerfeCTa Multiplex qPCR ToughMix Low ROX
Description
Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with co-amplification of four abundant (106) targets. PerfeCTa MultiPlex qPCR ToughMix enables multiplex qPCR assay performance comparable to single-plex qPCR probe assays without the need to rigorously titrate primer concentration. PerfeCTa MultiPlex qPCR ToughMix is a 5x concentrated, ready to-use reaction cocktail for real-time quantitative PCR (qPCR) with ToughMix reagent technology that neutralizes a broad spectrum of PCR inhibitors that compromise assay performance with crude extracts, clinical specimens, plant, soil environmental or complex food matrices. A key component of the kit is an ultrapure, highly-processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa MultiPlex qPCR ToughMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.
Details
Single-tube, 5X concentrated reagent containing:
- Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
- AccuStart II Taq DNA Polymerase.
- Proprietary enzyme stabilizers and performance-enhancing additives.
- Titrated reference dye (if applicable).
Instrument Compatibility
ROX
- Applied Biosystems 5700
- Applied Biosystems 7000
- Applied Biosystems 7300
- Applied Biosystems 7700
- Applied Biosystems 7900
- Applied Biosystems 7900HT
- Applied Biosystems 7900 HT Fast
- Applied Biosystems StepOne™
- Applied Biosystems StepOnePlus™
Low ROX
- Applied Biosystems 7500
- Applied Biosystems 7500 Fast
- Stratagene Mx3000P®
- Stratagene Mx3005P™
- Stratagene Mx4000™
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio™ (all models)
- Douglas Scientific IntelliQube®
No ROX
- Quantabio Q
- BioRad CFX
- Roche LightCycler 480
- QIAGEN Rotor-Gene Q
- Agilent AriaMx
- Azure Cielo™
- Analytik Jena qTower
- Analytik Jena qTOWERiris
- Other
Bio-Rad iCycler iQ systems
- BioRad iCycler iQ™
- BioRad MyiQ™
- BioRad iQ™5
Resources
Flyers
Product Manuals
Application Notes
CofA (PSFs)
SDSs
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PerfeCTa MultiPlex qPCR ToughMix
Although a small sample size was tested, the ToughMix Master Mix resulted in better sensitivity on our Campylobacter assay from fecal specimens (only 4 samples were tested, there appeared to be approximately 1 CT value decrease compared to our normal master mix TaqMan Fast Virus 1-Step Master Mix. Additionally, the PCR run time was able to be reduced by approximately 30 minutes and this product is cheaper. I plan on discussing with my PI about switching the SOP. Thank you!
Works perfectly with multiplex
The Perfecta qPCR TouchMix and Perfecta Multiplex qPCR Touchmix are two products tested in challenging conditions, with known PCR inhibitors present in the nucleic acid extract. Both mixes perform much better than conventional PCR Taq polymerases/buffers and also show clear improvement versus competing mastermixes. We saw a slight advantage for the Multiplex version for our multiplex assay versus the non-multiplex Perfecta qPCR Touchmix.