US

PerfeCTa MultiPlex qPCR ToughMix

Advanced 1-tube SuperMix optimized to support highly multiplexed DNA amplifications in miniaturized reaction volumes and withstand a broad spectrum of PCR inhibitors
Features & Benefits
  • 1-tube SuperMix minimizes pipetting steps, simplifying reaction assembly and improving accuracy
  • 5x concentrated reagent provides more flexibility with dilute DNA samples sensitivity
  • ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
  • Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
  • Supports efficient vortex mixing with proprietary anti-foaming additives

 

PerfeCTa Multiplex qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Product
Kit Size
Part Number
Price
Quantity
 
Product
Kit Size
Order Info
Price
Quantity

PerfeCTa Multiplex qPCR ToughMix

Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  250 x 25 μL rxns (1 x 1.25 mL)
Part Number:  95147-250
Price:  $570.00
Add to Cart
Kit Size:  1000 x 25 μL rxns (4 x 1.25 mL)
Part Number:  95147-01K
Price:  $2,150.00
Add to Cart
Kit Size:  5000 x 25 μL rxns (1 x 25 mL)
Part Number:  95147-05K
Price:  $9,409.00
Add to Cart
Product
Kit Size
Order Info
Price
Quantity

PerfeCTa Multiplex qPCR ToughMix ROX

Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  250 x 25 μL rxns (1 x 1.25 mL)
Part Number:  95148-250
Price:  $571.00
Add to Cart
Kit Size:  1000 x 25 μL rxns (4 x 1.25 mL)
Part Number:  95148-01K
Price:  $2,150.00
Add to Cart
Kit Size:  5000 x 25 μL rxns (1 x 25 mL)
Part Number:  95148-05K
Price:  $9,412.00
Add to Cart
Product
Kit Size
Order Info
Price
Quantity

PerfeCTa Multiplex qPCR ToughMix Low ROX

Request Sample
Kit Size:
Part Number:
Price:
Quantity:
Kit Size:  250 x 25 μL rxns (1 x 1.25 mL)
Part Number:  95149-250
Price:  $571.00
Add to Cart
Kit Size:  1000 x 25 μL rxns (4 x 1.25 mL)
Part Number:  95149-01K
Price:  $2,150.00
Add to Cart
Kit Size:  5000 x 25 μL rxns (1 x 25 mL)
Part Number:  95149-05K
Price:  $9,409.00
Add to Cart

Description

Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with co-amplification of four abundant (106) targets. PerfeCTa MultiPlex qPCR ToughMix enables multiplex qPCR assay performance comparable to single-plex qPCR probe assays without the need to rigorously titrate primer concentration. PerfeCTa MultiPlex qPCR ToughMix is a 5x concentrated, ready to-use reaction cocktail for real-time quantitative PCR (qPCR) with ToughMix reagent technology that neutralizes a broad spectrum of PCR inhibitors that compromise assay performance with crude extracts, clinical specimens, plant, soil environmental or complex food matrices. A key component of the kit is an ultrapure, highly-processive thermostable DNA polymerase that is free of detectable E. coli DNA. PerfeCTa MultiPlex qPCR ToughMix is ideal for demanding qPCR applications such as bacterial pathogen detection where residual host DNA in typical recombinant enzyme preparations can limit assay sensitivity and obscure detection of low copy samples.

Featured Publication

A Multiplexed Real-time PCR Assay to Detect SCID and SMN1 Homozygous Exon 7 Deletion, and A Droplet Digital PCR Assay to Assess SMN2 Copy Numbers, in Newborn screening for Spinal Muscular Atrophy

Download Featured Poster

Details

Details

Contents

Single-tube, 5X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
  • AccuStart II Taq DNA Polymerase.
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).
Instrument Capability

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5
Customer Testimonials

Customer Testimonials

PerfeCTa MultiPlex qPCR ToughMix

"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."

Bethany B. | Biotech company

Details

Contents

Single-tube, 5X concentrated reagent containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
  • AccuStart II Taq DNA Polymerase.
  • Proprietary enzyme stabilizers and performance-enhancing additives.
  • Titrated reference dye (if applicable).

Instrument Capability

ROX

  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™ (all models)
  • Douglas Scientific IntelliQube®

No ROX

  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Agilent AriaMx
  • Azure Cielo™
  • Other

Bio-Rad iCycler iQ systems

  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Resources

Flyers

Product Manuals

Application Notes

CofA (PSFs)

Click here to see all CofA (PSFs)

SDSs

Publications

Customer Testimonials

PerfeCTa MultiPlex qPCR ToughMix

"The Perfecta Multiplex qPCR ToughMix greatly simplified my qPCR assay by removing DNA isolation steps and complexity of passive reference dyes. I will definitely be using this product in the future."

Bethany B. | Biotech company

Welcome to the Quantabio webshop!

Please complete the new user account registration form

Once your account is setup, you'll be able to purchase any of the products on our site at any time with next day delivery for in stock products.

Product Finder

Select Your Assay

Starting Template

Assay Format

Detection Chemistry

Multiplexing (more than 3 targets)

Is gene-specific priming (GSP) required?

What current Reverse Transcriptase or cDNA kit are you using?

Select the group which contains your real-time PCR cycler

  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • QIAGEN Rotor-Gene Q
  • Azure Cielo 6
  • Azure Cielo 3
  • Other
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Choose your application from the categories below

Products

I give Quantabio or an authorized Quantabio distributor permission to contact me for product updates and news.
* Required information